Generator

Part:BBa_K1820021

Designed by: Sara Gertsch   Group: iGEM15_Utah_State   (2015-09-16)

P335-Φ31 inducible middle promoter + RBS + mCherry + term

A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the B0034 ribosomal binding site (RBS) (BBa_K1820001), a red fluorescent protein from the 2013 Uppsala team, previously termed mCherry, that was codon optimized for Lactobacillus reuteri (BBa_K1033250), and a transcriptional terminator.

Usage and Biology

This construct was designed to initiate transcription after phage infection of Lactococcus lactis, resulting in expression of mCherry (previously shown to have fluorescent activity in L. lactis). Designed to allow for fluorescent detection of Φ31 phage infection in L. lactis.

This construct has yet to be cloned and expressed in L. lactis, and validated for functionality.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42


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Categories
Parameters
None